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The BD OneFlow™ PCD Tube is a preconfigured, ready-to-use 8-color reagent provided in a single-test tube format. When run in parallel with the BD OneFlow™ PCST, it is intended for flow cytometric immunophenotyping of normal and aberrant plasma cells in bone marrow as an aid in the diagnosis of multiple myeloma or other plasma cell disorders.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

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The BD OneFlow™ PCST is a preconfigured, ready-to-use 8-color reagent comprising two tubes—one containing the cytoplasmic markers (C tube) and the other containing the surface markers (S tube). It is intended for flow cytometric immunophenotyping of normal polyclonal and aberrant plasma cell populations in bone marrow as an aid in the diagnosis of hematological disorders.

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BD OneFlow™ Reagents, BD FACSLyric™ Flow Cytometer with the BD FACSuite™ Clinical and BD FACSuite™ Applications, BD FACSCanto™ II Flow Cytometer, BD® FC Beads (8-Color, 7-Color, 5-Color and 2-Color Kits), BD OneFlow™ Setup Beads, BD FACSDiva™ CS&T IVD Beads, and BD® CS&T Beads are CE marked in compliance with the European In Vitro Diagnostic Medical Device Directive 98/79/EC.

Our innovative BD OneFlow™ Solution brings the standardization of leukemia and lymphoma immunophenotyping one step forward.

*This publication refers to EuroFlow and is relevant to the BD OneFlow™ Solution as the BD OneFlow™ Solution demonstrated results equivalent to EuroFlow through a clinical trial.

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Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

van Dongen JJM, Lhermitte L, Böttcher S, et al. on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708). EuroFlow antibody panels for standardised n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukaemia. 2012;26(9): 1908-1975. doi: 10.1038/leu.2012.120

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The BD OneFlow™ ALOT is a preconfigured, ready-to-use 8-color reagent comprising two tubes—one containing the cytoplasmic markers (C tube) and the other containing the surface markers (S tube). The tube is intended for flow cytometric immunophenotyping of aberrant immature populations of hematopoietic cells (lymphoid and non-lymphoid lineage) in bone marrow and peripheral blood as an aid in the diagnosis of acute lymphoblastic leukemia and non-lymphoid acute leukemia.

The BD OneFlow™ LST is a preconfigured, ready-to-use 8-color 12-antibodies reagent that is provided in a single-test tube format. It is intended for flow cytometric immunophenotyping of normal and aberrant mature lymphocyte populations of B, T and NK cell lineages in peripheral blood, bone marrow and lymph nodes as an aid in diagnosis of hematological disorders.

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The BD OneFlow™ B-CLPD T1 is a preconfigured, ready-to-use 8-color reagent provided in a single-test tube format. It shall be used for specimens with B cell lineage populations needing further investigation in combination with the BD OneFlow™ LST.  B-CLPD T1 is intended for flow cytometric immunophenotyping of B cells in peripheral blood and bone marrow as an aid in the diagnosis of chronic lymphocytic leukemia (CLL) and other B cell chronic lymphoproliferative diseases.

Moloney E, Watson H, Barge D, et al. Efficiency and health economic evaluations of BD OneFlow™ Flow Cytometry Reagents for diagnosing chronic lymphoid leukaemia. Cytometry B Clin Cytom. 2019;96(6):514-520. doi: 10.1002/cyto.b.21779

The EuroFlow trademark is the property of the EuroFlow Consortium and cannot be reproduced or published without prior written permission from the EuroFlow coordinator (www.euroflow.org).

Built on the research and validation work of the EuroFlow™ Consortium on the characterization of hematological malignancies for improved accurate diagnosis,1* the innovative BD OneFlow™ Solution brings the standardization of leukemia and lymphoma immunophenotyping one step forward, improving laboratory efficiency and enabling reliability and accuracy of results for clinical decisions and ultimately patient outcome.2, 3 The BD OneFlow™ Solution is a comprehensive set of reagents (LST, B-CLPD T1, PCST, PCD and ALOT), setup beads, protocols, and assay acquisition and analysis templates to reproducibly set up the flow cytometer and stain, acquire and analyze patient specimens for immunophenotyping of normal and aberrant cell populations.

van der Velden VHJ, Flores-Montero J, Peres-Andres M, et al. Optimisation and testing of dried antibody tube: The EuroFlow LST and PIDOT tubes as examples. J Immunol Methods. 2019;475:112287. doi: 10.1016/j.jim.2017.03.011